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(PDF) Architecture of the complete oxygen-sensing FixL-FixJ two ...
WebNov 1, 1995 · The R. leguminosarum bv. phaseoli FixL protein does not contain the heme-binding motif defined for the previously described FixL proteins. R. leguminosarum bv. phaseoli fixLJ and fixJ mutants were constructed. These mutants can still fix nitrogen, albeit at a reduced level. Expression analysis of nifA-gusA and nifH-gusA fusions in the ... http://www.reeis.usda.gov/web/crisprojectpages/0189546-tracking-the-oxygen-signal-in-the-fixlfixj-system-for-regulating-nitrogen-fixation.html cities in klickitat county washington
Functional analysis of the fixL fixJ and fixK genes in …
WebApr 11, 2024 · Wright et al., Sci. Signal. 11, eaa0825 (2024) 10 April 2024 SCIENCE SIGNALING RESEARCH ARTICLE 3 of 12 method, we obtained high-quality SAXS data from full-length FixL in both the met and the cyanomet forms. The plot of log q versus log I(q) obtained by SEC-SAXS at BL45XU (Fig. 2A) and the SEC- SAXS parameters are … WebApr 10, 2024 · The FixL and FixJ two-component system (TCS) of this bacterium ensures that nitrogen fixation is only stimulated under conditions of low oxygen. When it is not bound to oxygen, the histidine ... Webof FixL* from E. coli strainTG1 bearingpGG820 and plasmid pCHK57 has been described (16). The purification of FixJ from TG1 bear ing pCHK57 and pMW5 has been described (29). FixL*H138Q, FixL*H194N, and FixL*H285Q were purified exactly as was FixL*, except that the strain used for overexpression of these three plasmids was BL21 bearing … cities in klickitat county