Fl83b cells

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August undefined, 2024

WebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL … WebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N …

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WebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... WebDec 9, 2024 · FL83B cells were harvested at the exponential growth phase, prepared into a suspension of approximately 1 × 10 6 cells/mL, and 600 μL of this suspension was added to the lower chamber. FL83B cells were stimulated with 40 ng/mL TNF-α for 2 h in model group (+ TNF-α group). FL83B cells were stimulated with an equal volume of PBS for 2 … اسم میوه چهار نقطه ای

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WebMay 19, 2024 · FL83B cells were seeded in six-well plates at 4x10 5 cells per well with high-glucose DMEM containing 10 vol% FBS, penicillin-streptavidin-amphotericin B (Gibco … WebDec 29, 2015 · FL83B cell line represent normal hepatocytes whereas hHSC cell line has the angiotensin 1 receptors which is the main target for losartan receptors. Confocal … WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … cr judgment\\u0027s

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WebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ... WebCells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and … crju05-90-f8bWebJan 11, 2024 · d FL83B cells were cultured with different concentrations (as indicated) of PC (34:2) for 16 h, and the resulting effects on AhR expression were determined by western blots. Ratio to β-actin ... اسم میوه به عربی

"WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... " - Fl83b cells

Fl83b cells

Characterization of the mouse liver cell line FL83B

WebFeb 6, 2013 · FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, … WebMay 8, 2024 · The effects of a triterpene purified from bitter melon was investigated against TNF-α-induced inflammation via AMP-activated protein kinase in FL83B cells. This compound suppressed the TNF-α-induced expression of inflammatory markers, including inducible NO synthase, p65 subunit of NF-κB, TNF-α, and IL-1β (Cheng et al., 2012).

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WebJan 15, 2024 · FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes. WebJul 13, 2024 · Characteristics. tissue/cell type: Liver. condition: Healthy. treatment: none. Treatment protocol. FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non …

WebDec 1, 2024 · Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at …

WebOriginal magnification: 100×. (C) FL83B cells were transfected with HA-Daxx and then treated with or without TGF-β for 24 h. Western blotting analysis of Daxx, E-cadherin and Vimentin expression. Relative expression was normalized to β-actin expression as a reference. The graph shows quantitative densitometry from Western blotting. WebJan 1, 2024 · FL83B cells were grown in F12K medium supplemented with 10% foetal bovine serum (FBS)(Biological Industries, Haemek, Israel) and 100 mg/L penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. Cells induced lipid accumulation in hepatocytes using 0.5 mM oleic acid (OA) for 48 h, then 3–100 μM …

WebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the …

WebIn another experiment, FL83B cells were challenged with oleic acid to induce lipid accumulation and treated with various concentrations of fisetin. Results: NAFLD mice treated with fisetin had decreased body weight and epididymal adipose tissue weight compared to NAFLD mice. Fisetin treatment also reduced liver lipid droplet and … cr judo lazioWeb"KYO-1 Cells;急变期的慢性粒白血病活化克隆细胞 STR基因图谱 GD-C60408935 背景信息：详见相关文献介绍产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支) 【细胞培养中原虫的污染情况总结】原虫：培养液可轻微浑浊，显微镜下那些细小的点状 crjugateWebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ... اسم میوه ها انگلیسی به فارسیWebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … اسم میوه ها به انگلیسی با تلفظ فارسیWebA mouse HCC cell line (Hepa1-6) and a mouse hepatocyte line (FL83B) were used to elucidate the mechanism Cite 6th Dec, 2024 Mohamed Elkablawy Taibah University and … cr jug\\u0027sWebJul 1, 2024 · 2.2. Cell line and treatment. FL83B cells purchased from the Bioresource Collection and Research Center (BCRC, Taiwan). Cells were cultured in F12 medium (Invitrogen-Gibco™, Paisley, Scotland) added with 10% FBS, 2 mM glutamine, 1% penicillin and streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2.Cells … cr juddWebFAM83A-H are newly identified oncogenes characterised by a conserved DUF1669 domain. FAM83B can substitute for Ras to promote malignant transformation. Ablation of … اسم میوه یک نقطه ای