Hct116 p53+/+ cells
WebJul 20, 2024 · Isogenic human colorectal cancer cell lines HCT116 (p53 +/+ and p53-/-) were irradiated with high-LET carbon ions. Cell survival was determined by the standard … WebSep 9, 2024 · Cells (HCT116 WT, HCT116 p53 –/–, MCF7, and U2OS) were cultured at 37°C with 5% CO 2 in DMEM high glucose (Hyclone) supplemented with 5% fetal bovine serum (FBS, Hyclone) and penicillin–streptomycin. All cells used were obtained from ATCC, which performs short-tandem repeat profiling to confirm the identity of their cell lines.
Hct116 p53+/+ cells
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WebVectors expressing p53 R273H, p53 R248W, or control empty vector were transfected into HCT116 p53−/− or p53+/+ cells 24 hours prior to irradiation to 2 Gy. One hour after … WebHCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for …
WebMay 29, 2024 · HCT116 p53 −/− cells were plated in 96-well plates so they will be at 70–90% confluence at the time of transfection. DNA-lipid complexes were prepared in 10 μL/well Opti-MEM reduced serum medium (Gibco), using 0.1 μg/well plasmid DNA and 0.2 μL/well of P3000 and Lipofectamine 3000 reagents (Thermo Fischer, Germany). ... WebApr 7, 2024 · F Knockdown of DDX21 attenuates HCT116 cell ... Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nat Cell Biol. 2024 ...
WebJun 10, 2016 · Three biological replicates were used for p value, p*<0.01. (H,I) NGFR mRNA expression is inhibited by p53 knockdown. HCT116 p53+/+ (H) and H460 (I) cells were transfected with p53 or control siRNA for 72 hr, and Doxorubicin or 5-Fluorouracil was supplemented 15 hr before the cells were harvested for q-PCR (Mean ± SEM, n = 3). … WebFeb 27, 2004 · Exogenous p53 Responds to DNA Damage in p53 Knockout HCT116 Cells—Wild type p53 was cloned into a lentiviral vector under the control of the RSV …
WebJan 5, 2024 · The p53-null HCT116 p53 (-/-) cells were transduced HCT116 stable expressing p53 R273H and p53 R248W cell lines and were generated by transduction …
WebDec 2, 2005 · The inhibition of Rad51 foci observed in p53wt HCT116 cells correlates with our results reporting p53-dependent Rad51 downregulation in this cell line (Fig 2C). The specific contribution of p53-dependent mechanisms in repressing HR remains to be established; however, cooperative efficient repression of HR by p53 may be required. towngas service pledgeWebEnter the email address you signed up with and we'll email you a reset link. towngas shareWebFurthermore, downregulation of JMJD2D inhibited cell proliferation in wild-type and even more so in p53(-/-) HCT116 colon cancer cells, suggesting that JMJD2D is a pro … towngas sgsWebSep 9, 2005 · Because Bcl-xL expression remains constant after UV treatment in HCT116 cells (Fig. 2A and figs. S2C and S3A), we hypothesized that if large amounts of stabilized p53 accumulate, a fraction of the cytosolic p53 might remain unassociated with Bcl-xL, eliminating the requirement for PUMA to release cytosolic p53 from Bcl-xL. towngas service centreWebJan 12, 2024 · Wild-type HCT116 cells (HCT116/p53 +/+) and p53 knockout HCT116 cells (HCT116/p53 −/−) were a gift from Dr. Yao Lin (Fujian Normal University, Fujian, China) and originally obtained from Dr Bert Vogelstein (Johns Hopkins University, Baltimore, MD). All human cell lines have been authenticated using STR (or SNP) profiling within the last 3 ... towngas sharepointWebAug 25, 2009 · The use of p53-specific siRNA in HCT116 (p53 +/+) cells also elicited the same divergent pattern of p53 transcriptional behavior, where Lasp1 mRNA was up … towngas shopWebDec 2, 2005 · The inhibition of Rad51 foci observed in p53wt HCT116 cells correlates with our results reporting p53-dependent Rad51 downregulation in this cell line (Fig 2C). The … towngas shop location